Doubled Haploids via Microspore Embryogenesis in Sugarbeet and Red Beet

Beet microspore embryogenesis and doubled haploid production technology are not available so far, and the only promising results were obtained in the founder?s laboratory. Micro-Crop continues the research program to establish beet ME and DH production and technology is expected to be ready for large scale use by the end of the second or even third year of company‘s business activity. Established technology will be subject for IP and will be licensed on a non-exclusive basis to interested companies and also used for custom production of beet homozygous lines.

a) State of art of sugarbeet doubled haploids: Sugarbeet doubled haploid production is well established via ovule culture (gynogenesis). Although the method is routinely used in companies, high cost, low efficiency and necessity to diploidize the obtained haploids makes it a potential setback in today’s technology. Therefore, an alternative could be to double the haploid production with microspore embryogenesis. Until recently, even isolation of viable population of sugarbeet microspores had been a problem. The same situation is in other beets (red beet, for example).

b) Progress, made in MicroCrop: MicroCrop achieved great progress in sugarbeet microspore embryogenesis and doubled haploid production using this technology. Careful staging and optimization of culture media allowed isolation of sugarbeet microspores in vitro culture at high viablity and vigour. Isolated and cultured microspores divide with formation of multicellular structures and calli. At present, attempts are made to regenerate plants from obtained calli.




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