Doubled Haploids via Microspore Embryogenesis in Cotton

Doubled haploid production is not established in this crop so far by any means (gynogenesis, distance hybridization, microspore embryogenesis) despite semigamy and polyembryony, described in cotton. MicroCrop researchers achieved great progress in cotton microspore embryogenesis and doubled haploid production. Careful staging and optimization of culture media allowed to isolate and in vitro culture of cotton microspores at high viability and vigor (see Fig 1 - 2). For the first time, Isolated and cultured cotton microspores divided with formation of multicellular structures and pro-embryo (see Fig 3). At present attempts are made to regenerate plants from gained pro-embryos and increase the frequency of sporophytic divisions.

Micro-Crop continues the research program to establish cotton ME and DH production and technology is expected to be ready for large scale use by the end of the second or third year of company’s business activity. Established technology will be subject for IP and will be licensed to interested companies and also used for custom production of cotton doubled haploids and homozygous lines.

 

Figure 1: Cotton male gametophyte development

 

Figure 2: Star-like, embryogenic cotton microspores under the light microscope and after FDA stainingt

 

Figure 3: Sporophytic division of cotton microspores in culture and formation of pro-embryo