Doubled Haploids via Microspore Embryogenesis in Brassica

Brassica napus is a species, where microspore embryogenesis and doubled haploid production is rather routine, and used for commercial production of doubled haploids in many seed companies. The main problems of technology are a) not all genotypes are responsive; b) very low frequency of spontaneous doubled haploids (less than 15% from total regenerated plants); c) rather low regeneration efficiency (many callus-like structures and secondary embryogenesis).

MicroCrop achieved some progress in solving these problems: a) novel antimitotic drugs are tested in Brassica and results are promising (up to 70% plants are DH, when microspores are cultured in the presence of drug); b) novel method – microspore embryogenesis from suspensor like structures, originated from cultured microspores turned to be a) genotype independent and b) very efficient in the quality of embryos and their regeneration efficiency (see Fig. below).

Similar approaches are used to obtain doubled haploids in Brasica oleraceae L, which is rather recalcitrant as preliminary data shows, that microspore embryogenesis from suspensor like structures can be an efficient alternative and adapted to the commercial production of DH in this species.

MicroCrop is ready to collaborate with seed companies on the custom based production of doubled haploids in various Brassica species and varieties. The company is also ready to do research to further develop DH protocol in Brassica and other related vegetables.

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